PRE-CONFERENCE WORKSHOP DAY

Workshop A

Discussing Treg Cell-based Therapy Challenges & Exploring How to Improve the Manufacturing Process

 

The development of Treg cell-based immunotherapies requires the effective ex vivo expansion to achieve the required numbers of clinical competent Treg cells. The standard procedure involves the isolation of suppressor T cells in a closed system of cell processing to enrich CD25+ T cells. Once isolated, most protocols use anti-CD3/ anti-CD28 beads and IL-2 in combination with Rapamycin to induce expansion of Tregs and prevent the outgrowth of CD25+ effector T cells. The last step is the collection of the expanded Treg cells free of the stimulating beads for infusion. We will discuss the challenges of each step and will address potential approaches to improve the manufacturing process.

Attend this workshop to:

  • Learn from our extensive clinical experience in transplantation
  • Discuss the challenges of the manufacturing process: purification, expansion, timelines, dosage and functional standards of Treg cells
  • Discover potential novel approaches to improve current standards
Workshop Leaders:
Francesc Marti

Francesc Marti
Associate Professor
University of Kentucky

Roberto Gedaly

Roberto Gedaly
Professor
University of Kentucky

Workshop A

Purification & Expansion of Regulatory T Cells

 

Clinical application of Tregs has historically been hampered by their unfavorable ex vivo expansion properties. This workshop will discuss and introduce techniques for purification and expansion of regulatory T cells.

 

This workshop will cover:

1. Introduction
2. Purification of Tregs Using Clini-MACS
3. Alternative Purification Methods – Flow Sorting
4. Quality Control of the Purified Treg Product
5. Polyclonal Expansion Protocol Used at
Northwestern
6. Polyclonal Expansion Protocols Used Elsewhere
7. Antigen Specific Treg Expansion Protocols
8. Bead Removal from Expanded Treg Products
9. Sterility Testing of Expanded Tregs
10. Functional Testing of Expanded Tregs
11. Clinical Experiences with the Expanded Treg Products
12. Post-infusion Immune Monitoring of
Patients
13. Concluding Remarks
Workshop Leaders:
James Mathew

James Mathew
Professor
Northwestern University

joseph

Joseph Leventhal
Professor
Northwestern University